{"id":1366,"date":"2024-10-13T14:33:34","date_gmt":"2024-10-13T14:33:34","guid":{"rendered":"https:\/\/novanta.com\/fr\/precision-manufacturing\/resources\/whitepapers\/impulsions-ultra-courtes-en-microscopie-a-deux-photons\/"},"modified":"2026-09-02T11:01:35","modified_gmt":"2026-09-02T11:01:35","slug":"impulsions-ultracourtes-en-microscopie-a-deux-photons","status":"publish","type":"novanta_tech_paper","link":"https:\/\/novanta.com\/fr\/precision-manufacturing\/ressources\/documents-blancs\/impulsions-ultracourtes-en-microscopie-a-deux-photons\/","title":{"rendered":"Les avantages des impulsions ultracourtes en microscopie \u00e0 deux photons"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-technical-paper-overview\">Pr\u00e9sentation de l&rsquo;article technique<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-choosing-an-ultrashort-pulse-laser-system-for-two-photon-microscopy-pulse-duration-at-the-sample-not-at-the-laser-output-decides-tpm-benefit\"><strong>Choix d&rsquo;un syst\u00e8me laser \u00e0 impulsions ultracourtes pour la microscopie \u00e0 deux photons<\/strong><br><em>C&rsquo;est la dur\u00e9e d&rsquo;impulsion au niveau de l&rsquo;\u00e9chantillon, et non \u00e0 la sortie du laser, qui d\u00e9termine les avantages de la microscopie \u00e0 deux photons (TPM).<\/em><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">La microscopie \u00e0 deux photons stagne depuis pr\u00e8s de trente ans sur une enveloppe d\u2019excitation de 100 \u00e0 150 fs \/ 80 \u00e0 100 MHz \u2014 non pas parce que les impulsions inf\u00e9rieures \u00e0 10 fs n\u2019apportent aucun avantage (elles le font bel et bien : imagerie plus profonde, rendement de fluorescence plus \u00e9lev\u00e9, excitation de plusieurs fluorophores sans r\u00e9glage, moins de photod\u00e9gradation), mais parce que chaque \u00e9l\u00e9ment optique suppl\u00e9mentaire entre le laser et l\u2019\u00e9chantillon allonge l\u2019impulsion. Au moment o\u00f9 une impulsion inf\u00e9rieure \u00e0 10 fs atteint le plan focal \u00e0 travers un objectif standard de 63\u00d7, la dispersion a d\u00e9truit la majeure partie de ce que le laser avait fourni. R\u00e9sultat : la plupart des laboratoires de sciences de la vie ne disposent pas d\u2019un ing\u00e9nieur en photonique sur place pour g\u00e9rer la pr\u00e9compensation par miroir chirp\u00e9 ; ils se rabattent donc par d\u00e9faut sur des impulsions plus s\u00fbres de 150 fs et s\u2019accommodent de la limite de profondeur d\u2019imagerie.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Cet article technique explique comment le logiciel de mod\u00e9lisation de la dispersion venteon ultra de Novanta (&lt; 5,5 fs mesur\u00e9s), le logiciel de mod\u00e9lisation de la dispersion \u00ab venteon vChirp \u00bb, le jeu de miroirs chirp\u00e9s DCM et l\u2019unit\u00e9 de caract\u00e9risation d\u2019impulsion + compression automatique \u00ab d-scan \u00bb de Novanta fonctionnent comme un syst\u00e8me en boucle ferm\u00e9e qui d\u00e9livre <strong>des impulsions de 6,4 fs FWHM au niveau du plan de l\u2019\u00e9chantillon<\/strong> \u00e0 travers un objectif standard de 63\u00d7, NA de 0,75 \u2014 sans n\u00e9cessiter d\u2019expertise en photonique au niveau du microscope.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-key-takeaways-include\">Principaux points \u00e0 retenir :<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Pourquoi les impulsions inf\u00e9rieures \u00e0 10 fs sont rest\u00e9es l\u2019apanage des sp\u00e9cialistes pendant 30 ans \u2014 la dispersion dans le train optique du microscope d\u00e9truit ce que le laser fournit<\/li>\n\n\n\n<li>Comment les technologies vChirp et d-scan bouclent la boucle : le logiciel mod\u00e9lise le trajet du faisceau, la caract\u00e9risation mesure l\u2019impulsion au niveau de l\u2019\u00e9chantillon, et l\u2019auto-compression s\u2019ajuste en temps r\u00e9el<\/li>\n\n\n\n<li>Quand privil\u00e9gier le syst\u00e8me venteon ultra par rapport \u00e0 un Ti:Sapphire standard de 100 fs : imagerie en profondeur des tissus, excitation multi-fluorophore, exp\u00e9riences sur cellules vivantes sensibles aux photodommages<\/li>\n\n\n\n<li>Ce qu\u2019offre le Venteon Ultra : impulsions limit\u00e9es par transformation &lt; 5 fs \/ mesur\u00e9es &lt; 5,5 fs, sortie spectrale large couvrant toute la gamme d\u2019\u00e9mission du Ti:Sapphire<\/li>\n\n\n\n<li>Un flux de travail pratique : saisir les \u00e9l\u00e9ments optiques du trajet du faisceau dans vChirp \u2192 pr\u00e9dire la configuration des miroirs chirp\u00e9s \u2192 effectuer la mesure avec d-scan au niveau de l\u2019\u00e9chantillon \u2192 compenser automatiquement<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"h-\">T\u00e9l\u00e9chargez l\u2019article technique complet pour d\u00e9couvrir le sch\u00e9ma du train optique avec la boucle de compression automatique du d-scan, la logique de mod\u00e9lisation de la dispersion sur laquelle repose vChirp, ainsi que le r\u00e9sultat mesur\u00e9 de 6,4 fs FWHM apr\u00e8s un objectif standard \u2014 avant de sp\u00e9cifier une source TPM de 100 fs et de limiter votre profondeur d\u2019imagerie, votre contraste et votre plage multifluorophore \u00e0 cause d\u2019une dispersion que vous auriez pu compenser.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pr\u00e9sentation de l&rsquo;article technique Choix d&rsquo;un syst\u00e8me laser \u00e0 impulsions ultracourtes pour la microscopie \u00e0 deux photonsC&rsquo;est la dur\u00e9e d&rsquo;impulsion au niveau de l&rsquo;\u00e9chantillon, et non \u00e0 la sortie du laser, qui d\u00e9termine les avantages de la microscopie \u00e0 deux photons (TPM). La microscopie \u00e0 deux photons stagne depuis pr\u00e8s de trente ans sur une [&hellip;]<\/p>\n","protected":false},"author":342,"featured_media":353,"template":"","meta":{"_acf_changed":true,"card_thumbnail_id":0,"show_table_of_content":true},"class_list":["post-1366","novanta_tech_paper","type-novanta_tech_paper","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.1 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Ultrashort Pulses in Two-Photon Microscopy | Novanta<\/title>\n<meta name=\"description\" content=\"Ce livre blanc explique comment les impulsions laser ultracourtes am\u00e9liorent la profondeur et la r\u00e9solution de la microscopie \u00e0 deux photons dans le cadre d&#039;applications biom\u00e9dicales d&#039;imagerie laser.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" 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