{"id":1368,"date":"2024-10-14T16:51:45","date_gmt":"2024-10-14T16:51:45","guid":{"rendered":"https:\/\/novanta.com\/fr\/precision-manufacturing\/resources\/whitepapers\/pinces-laser-spectroscopie-raman\/"},"modified":"2026-09-02T11:01:40","modified_gmt":"2026-09-02T11:01:40","slug":"pinces-laser-spectroscopie-raman","status":"publish","type":"novanta_tech_paper","link":"https:\/\/novanta.com\/fr\/precision-manufacturing\/ressources\/documents-blancs\/pinces-laser-spectroscopie-raman\/","title":{"rendered":"Spectroscopie Raman par pince laser (LTRS)"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-technical-paper-overview\">Pr\u00e9sentation de l&rsquo;article technique<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-laser-tweezers-raman-spectroscopy-single-cell-cancer-diagnostics-microfluidic-flow-control-not-operator-skill-decides-whether-ltrs-scales-clinical\"><strong>Spectroscopie Raman par pince laser : diagnostic du cancer au niveau de la cellule unique<\/strong><br><em>C&rsquo;est le contr\u00f4le microfluidique du d\u00e9bit, et non les comp\u00e9tences de l&rsquo;op\u00e9rateur, qui d\u00e9termine si la LTRS peut \u00eatre transpos\u00e9e \u00e0 l&rsquo;\u00e9chelle clinique.<\/em><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">L\u2019analyse de tissus en vrac gomme justement les caract\u00e9ristiques propres \u00e0 chaque cellule qui importent le plus pour le diagnostic du cancer et les \u00e9tudes de r\u00e9ponse aux m\u00e9dicaments. La spectroscopie Raman est une technique sans marquage et non destructive \u2014 id\u00e9ale pour les cellules vivantes \u2014 mais la diffusion Raman est peu efficace, la collecte du signal prend plusieurs secondes et les cellules doivent rester parfaitement immobiles pendant l\u2019acquisition. Les bancs d\u2019essai traditionnels combinant pi\u00e8ge optique et spectroscopie Raman n\u00e9cessitent une intervention constante de l\u2019op\u00e9rateur, ce qui emp\u00eache le passage du laboratoire de recherche au flux de travail clinique.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Cet article technique pr\u00e9sente une configuration LTRS automatis\u00e9e combinant une chambre d\u2019\u00e9coulement microfluidique \u00e0 faible co\u00fbt, une pompe \u00e0 seringue et des lasers Novanta Laser Quantum ventus \u00e0 double longueur d\u2019onde \u2014 ventus 1064 (TEM00, pr\u00e9cision M\u00b2 de 95 %) pour des acquisitions en 2 secondes.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-key-takeaways-include\">Principaux points \u00e0 retenir :<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Pourquoi la spectroscopie Raman sur tissu en vrac passe \u00e0 c\u00f4t\u00e9 de ce que la LTRS unicellulaire d\u00e9tecte : les signatures du cytochrome c et des acides nucl\u00e9iques qui distinguent les cellules tumorales circulantes des globules blancs<\/li>\n\n\n\n<li>Fonctionnement du LTRS \u00e0 double longueur d\u2019onde : la longueur d\u2019onde de 1 064 nm pi\u00e8ge les cellules dans un gradient optique de l\u2019ordre du pico-Newton ; celle de 532 nm excite les bandes Raman r\u00e9sonantes permettant de diagnostiquer l\u2019\u00e9tat pathologique<\/li>\n\n\n\n<li>Quand privil\u00e9gier le LTRS \u00e0 double longueur d\u2019onde plut\u00f4t que le LTRS \u00e0 laser unique : discrimination de niveau clinique, \u00e9tudes d\u2019interaction m\u00e9dicament-cellule, stadification du cancer de la prostate \u00e0 un stade avanc\u00e9<\/li>\n\n\n\n<li>Ce qu\u2019offre la paire Ventus : faisceau de pi\u00e9geage M\u00b2 &lt; 1,4, largeur de raie Raman de 1 cm\u207b\u00b9, stabilit\u00e9 de pointage &lt; 10 \u03bcrad\/\u00b0C sur les deux longueurs d\u2019onde, contr\u00f4le via RS232 + LabVIEW<\/li>\n\n\n\n<li>Un flux de travail pratique : chambre d\u2019\u00e9coulement microfluidique + pompe \u00e0 seringue + paire Ventus co-align\u00e9e \u2192 capture automatis\u00e9e de cellules individuelles et acquisition de spectres<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"h-\">T\u00e9l\u00e9chargez le document technique complet pour d\u00e9couvrir la configuration optique exp\u00e9rimentale du LTRS, les spectres Raman PC-3 vs Jurkat montrant la bande de discrimination du cytochrome c, ainsi que les r\u00e9sultats de classification par analyse discriminante lin\u00e9aire \u2014 avant d\u2019opter pour un syst\u00e8me LTRS \u00e0 longueur d\u2019onde unique qui contraindra votre \u00e9quipe \u00e0 une capture manuelle des cellules et limitera la vitesse d\u2019acquisition.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pr\u00e9sentation de l&rsquo;article technique Spectroscopie Raman par pince laser : diagnostic du cancer au niveau de la cellule uniqueC&rsquo;est le contr\u00f4le microfluidique du d\u00e9bit, et non les comp\u00e9tences de l&rsquo;op\u00e9rateur, qui d\u00e9termine si la LTRS peut \u00eatre transpos\u00e9e \u00e0 l&rsquo;\u00e9chelle clinique. L\u2019analyse de tissus en vrac gomme justement les caract\u00e9ristiques propres \u00e0 chaque cellule qui [&hellip;]<\/p>\n","protected":false},"author":342,"featured_media":1055,"template":"","meta":{"_acf_changed":false,"card_thumbnail_id":0,"show_table_of_content":true},"class_list":["post-1368","novanta_tech_paper","type-novanta_tech_paper","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.1 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Laser Tweezers Raman Spectroscopy | Novanta<\/title>\n<meta name=\"description\" content=\"D\u00e9couvrez comment les pinces laser s&#039;associent \u00e0 la spectroscopie Raman dans les syst\u00e8mes laser destin\u00e9s \u00e0 la recherche scientifique pour pi\u00e9ger et analyser des particules ou des cellules individuelles.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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