{"id":1372,"date":"2024-10-14T16:51:45","date_gmt":"2024-10-14T16:51:45","guid":{"rendered":"https:\/\/novanta.com\/precision-manufacturing\/?post_type=novanta_tech_paper&#038;p=1372"},"modified":"2026-07-24T15:48:39","modified_gmt":"2026-07-24T15:48:39","slug":"laser-tweezers-raman-spectroscopy","status":"publish","type":"novanta_tech_paper","link":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/","title":{"rendered":"Laser Tweezers Raman Spectroscopy (LTRS)"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-technical-paper-overview\">Technical Paper Overview<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-laser-tweezers-raman-spectroscopy-single-cell-cancer-diagnostics-microfluidic-flow-control-not-operator-skill-decides-whether-ltrs-scales-clinical\"><strong>Laser Tweezers Raman Spectroscopy: Single-Cell Cancer Diagnostics<\/strong><br><em>Microfluidic flow control, not operator skill, decides whether LTRS scales clinical.<\/em><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Bulk tissue analysis averages out the very single-cell features that matter most for cancer diagnostics and drug-response studies. Raman spectroscopy is label-free and non-destructive \u2014 ideal for living cells \u2014 but Raman scattering is inefficient, signal collection takes seconds, and cells must be held perfectly still during acquisition. Traditional optical-trap-plus-Raman benches require constant operator intervention, blocking translation from research lab to clinical workflow.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This technical paper documents an automated LTRS arrangement combining a low-cost microfluidic flow chamber, a syringe pump, and dual-wavelength Novanta Laser Quantum ventus lasers \u2014 ventus 1064 (TEM00, M\u00b2 95% accuracy in 2-second acquisitions.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-key-takeaways-include\">Key takeaways include:<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Why bulk tissue Raman misses what single-cell LTRS catches: cytochrome-c and nucleic-acid signatures that distinguish circulating tumour cells from white blood cells<\/li>\n\n\n\n<li>How dual-wavelength LTRS works: 1064 nm traps cells in a pico-Newton optical gradient; 532 nm excites resonant Raman bands diagnostic of disease state<\/li>\n\n\n\n<li>When to specify dual-wavelength over single-laser LTRS: clinical-grade discrimination, drug-cell interaction studies, late-stage prostate cancer staging<\/li>\n\n\n\n<li>What the ventus pair delivers: M\u00b2 <1.4 trap beam, 1 cm\u207b\u00b9 Raman line width, <10 \u03bcrad\/\u00b0C pointing stability across both wavelengths, RS232 + LabVIEW control<\/li>\n\n\n\n<li>A practical workflow: microfluidic flow chamber + syringe pump + co-aligned ventus pair \u2192 automated single-cell capture and spectrum acquisition<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\" id=\"h-\">Download the full technical paper for the experimental LTRS optical layout, the PC-3 vs. Jurkat Raman spectra showing the cytochrome-c discrimination band, and the Linear Discriminate Analysis classification results \u2014 before specifying a single-wavelength LTRS bench that locks your team into manual cell-capture and limits acquisition speed.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Technical Paper Overview Laser Tweezers Raman Spectroscopy: Single-Cell Cancer DiagnosticsMicrofluidic flow control, not operator skill, decides whether LTRS scales clinical. Bulk tissue analysis averages out the very single-cell features that matter most for cancer diagnostics and drug-response studies. Raman spectroscopy is label-free and non-destructive \u2014 ideal for living cells \u2014 but Raman scattering is inefficient, [&hellip;]<\/p>\n","protected":false},"author":342,"featured_media":4443,"template":"","meta":{"_acf_changed":false,"card_thumbnail_id":0,"show_table_of_content":true},"class_list":["post-1372","novanta_tech_paper","type-novanta_tech_paper","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Laser Tweezers Raman Spectroscopy | Novanta<\/title>\n<meta name=\"description\" content=\"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Laser Tweezers Raman Spectroscopy (LTRS)\" \/>\n<meta property=\"og:description\" content=\"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"Precision Manufacturing\" \/>\n<meta property=\"article:modified_time\" content=\"2026-07-24T15:48:39+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/04\/Fig_2-562x395-1.png?quality=85&strip=all&w=562&h=395&crop=1\" \/>\n\t<meta property=\"og:image:width\" content=\"562\" \/>\n\t<meta property=\"og:image:height\" content=\"395\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/\",\"url\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/\",\"name\":\"Laser Tweezers Raman Spectroscopy | Novanta\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/wp-content\\\/uploads\\\/sites\\\/28\\\/2026\\\/04\\\/Fig_2-562x395-1.png\",\"datePublished\":\"2024-10-14T16:51:45+00:00\",\"dateModified\":\"2026-07-24T15:48:39+00:00\",\"description\":\"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/#primaryimage\",\"url\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/wp-content\\\/uploads\\\/sites\\\/28\\\/2026\\\/04\\\/Fig_2-562x395-1.png\",\"contentUrl\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/wp-content\\\/uploads\\\/sites\\\/28\\\/2026\\\/04\\\/Fig_2-562x395-1.png\",\"width\":562,\"height\":395},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/resources\\\/whitepapers\\\/laser-tweezers-raman-spectroscopy\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Laser Tweezers Raman Spectroscopy (LTRS)\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#website\",\"url\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/\",\"name\":\"Precision Manufacturing\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#organization\",\"name\":\"Precision Manufacturing\",\"url\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/wp-content\\\/uploads\\\/sites\\\/28\\\/2026\\\/05\\\/novanta-icon-512x512-1.png?quality=85&strip=all\",\"contentUrl\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/wp-content\\\/uploads\\\/sites\\\/28\\\/2026\\\/05\\\/novanta-icon-512x512-1.png?quality=85&strip=all\",\"width\":512,\"height\":512,\"caption\":\"Precision Manufacturing\"},\"image\":{\"@id\":\"https:\\\/\\\/novanta.com\\\/precision-manufacturing\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Laser Tweezers Raman Spectroscopy | Novanta","description":"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/","og_locale":"en_US","og_type":"article","og_title":"Laser Tweezers Raman Spectroscopy (LTRS)","og_description":"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.","og_url":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/","og_site_name":"Precision Manufacturing","article_modified_time":"2026-07-24T15:48:39+00:00","og_image":[{"url":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/04\/Fig_2-562x395-1.png?quality=85&strip=all&w=562&h=395&crop=1","width":562,"height":395,"type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/","url":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/","name":"Laser Tweezers Raman Spectroscopy | Novanta","isPartOf":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/#website"},"primaryImageOfPage":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/#primaryimage"},"image":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/#primaryimage"},"thumbnailUrl":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/04\/Fig_2-562x395-1.png","datePublished":"2024-10-14T16:51:45+00:00","dateModified":"2026-07-24T15:48:39+00:00","description":"Discover how laser tweezers combine with Raman spectroscopy in scientific research laser systems to trap and analyze single particles or cells.","breadcrumb":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/#primaryimage","url":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/04\/Fig_2-562x395-1.png","contentUrl":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/04\/Fig_2-562x395-1.png","width":562,"height":395},{"@type":"BreadcrumbList","@id":"https:\/\/novanta.com\/precision-manufacturing\/resources\/whitepapers\/laser-tweezers-raman-spectroscopy\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/novanta.com\/precision-manufacturing\/"},{"@type":"ListItem","position":2,"name":"Laser Tweezers Raman Spectroscopy (LTRS)"}]},{"@type":"WebSite","@id":"https:\/\/novanta.com\/precision-manufacturing\/#website","url":"https:\/\/novanta.com\/precision-manufacturing\/","name":"Precision Manufacturing","description":"","publisher":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/novanta.com\/precision-manufacturing\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/novanta.com\/precision-manufacturing\/#organization","name":"Precision Manufacturing","url":"https:\/\/novanta.com\/precision-manufacturing\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/novanta.com\/precision-manufacturing\/#\/schema\/logo\/image\/","url":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/05\/novanta-icon-512x512-1.png?quality=85&strip=all","contentUrl":"https:\/\/novanta.com\/precision-manufacturing\/wp-content\/uploads\/sites\/28\/2026\/05\/novanta-icon-512x512-1.png?quality=85&strip=all","width":512,"height":512,"caption":"Precision Manufacturing"},"image":{"@id":"https:\/\/novanta.com\/precision-manufacturing\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/novanta_tech_paper\/1372","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/novanta_tech_paper"}],"about":[{"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/types\/novanta_tech_paper"}],"author":[{"embeddable":true,"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/users\/342"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/media\/4443"}],"wp:attachment":[{"href":"https:\/\/novanta.com\/precision-manufacturing\/wp-json\/wp\/v2\/media?parent=1372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}