{"id":172,"date":"2020-10-21T09:20:25","date_gmt":"2020-10-21T09:20:25","guid":{"rendered":"https:\/\/novanta.com\/precision-manufacturing\/?p=172"},"modified":"2026-07-23T16:44:55","modified_gmt":"2026-07-23T16:44:55","slug":"how-to-choose-a-raman-spectroscopy-laser","status":"publish","type":"post","link":"https:\/\/novanta.com\/precision-manufacturing\/articles\/how-to-choose-a-raman-spectroscopy-laser\/","title":{"rendered":"How to Choose a Raman Spectroscopy Laser"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Raman spectroscopy is a non-destructive analytical technique that yields detailed information about the chemical structure, crystallinity, and phase of sample matter. It can also be used to investigate various molecular interactions based on the scattering of incident light. Lasers have proven incredibly valuable as light sources for Raman scattering due to their high intensity and practical monochromaticity. But not all Raman spectroscopy\u00a0<a href=\"https:\/\/novantaphotonics.wistia.com\/medias\/wkhpeih1jd\" target=\"_blank\" rel=\"noreferrer noopener\">lasers<\/a>\u00a0are created equally. So, what parameters should you consider when selecting a laser for Raman spectroscopy? \u00a0<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-considering-excitation-wavelength\"><strong>Considering Excitation Wavelength<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Molecules typically scatter most incoming laser light at the same wavelength as that of the source. This is known as Rayleigh scattering. A much smaller portion of the beam \u2013 as little as 0.0000001% \u2013 is scattered at a different wavelength. This is Raman scattering, and it is this comparatively weak phenomenon that provides insights into the analyte\u2019s chemical structure. &nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-raman-intensity\"><strong>Raman Intensity<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">The intensity of Raman scattering is tied directly to the excitation wavelength. Generally, Raman spectroscopy lasers with longer wavelengths in the near-infrared (NIR) region correlate to a relatively weak scattering signal. Conversely, short excitation wavelengths typically give stronger signals. An ultraviolet (UV) excitation channel may yield a Raman intensity that is several orders of magnitude greater than that of a NIR laser, for instance. &nbsp; Excitation wavelength-dependent Raman scattering means that NIR Raman spectroscopy lasers usually require longer acquisition times and greater accumulation numbers. Each of these values decreases exponentially for lasers with visible and sub-visible excitation channels. &nbsp; However, molecules excited by laser light on the UV-visible spectrum often emit fluorescence that is much stronger than the Raman effect. This is known as fluorescence background, and it is a common pain point for Raman spectroscopy laser sources. &nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-fluorescence-background\"><strong>Fluorescence Background<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Background fluorescence is unwanted noise that can arise from a variety of sources, including the excited sample, the substrate, and the optical elements. Fluorescence essentially drowns out weaker signals and makes it difficult to acquire clear Raman spectra, particularly over longer acquisition scans where background fluorescence can saturate the detector. &nbsp; Fluorescence is an intrinsic process based on absorption. Fewer molecules absorb in the visible compared to the ultraviolet regions \u2013 likewise for near-infrared compared to visible light. Raman spectroscopy lasers with longer wavelengths are subsequently preferred for samples known to suffer from high fluorescence background. However, the trade-off in Raman intensity is significant, so there is usually a compromise. &nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-typical-excitation-wavelengths\"><strong>Typical Excitation Wavelengths<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">There are benefits and drawbacks to choosing either short or long-wave excitation signals which are typically dictated by sample type. High energy lasers can damage sample materials, but lower energy light sources require longer exposure times which can prove just as damaging. Consequently, the most common lasers used to acquire detailed Raman spectra without damaging samples are 532nm and 785nm light sources. &nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-raman-spectroscopy-light-sources-from-laser-quantum\"><strong>Raman Spectroscopy Light Sources from Laser Quantum<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">At Laser Quantum, we offer a series of lasers suitable for Raman spectroscopy light sources. Our foremost product offering is the ventus 532, an industry-leading laser for Raman spectroscopy that is unrivalled in terms of its size-to-power ratio. This is our most compact 532nm laser and it is optimised for success in the scientific industry. &nbsp; If you are looking for a visible&nbsp;<a href=\"https:\/\/novantaphotonics.com\/product\/ventus-solid-state-continuous-wave-lasers\/\" target=\"_blank\" rel=\"noreferrer noopener\">Raman spectroscopy laser<\/a>, or a light source extending into the infrared region, contact a member of our team today.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Raman spectroscopy is a non-destructive analytical technique that yields detailed information about the chemical structure, crystallinity, and phase of sample matter. It can also be used to investigate various molecular interactions based on the scattering of incident light. Lasers have proven incredibly valuable as light sources for Raman scattering due to their high intensity and [&hellip;]<\/p>\n","protected":false},"author":342,"featured_media":4046,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"card_thumbnail_id":0,"show_table_of_content":true,"footnotes":""},"categories":[171,158],"tags":[187],"class_list":["post-172","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-article","category-articles","tag-laser-sources"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.0 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>How to Choose a Raman Spectroscopy Laser | Novanta<\/title>\n<meta name=\"description\" content=\"Learn the key wavelength and power factors for choosing a Raman spectroscopy laser suited to your sample type and confocal microscopy 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